Design, synthesis and 64Cu labeling of fatty acid analogs containing dithiosemicarbazone chelate

Area of Science:

• Radiochemistry
• Organic Synthesis
• Nuclear Medicine

Background:

• Development of radiotracers for myocardial imaging is crucial.
• Fatty acid analogs are potential candidates for imaging myocardial metabolism.
• Copper-62 (62Cu) offers favorable properties for positron emission tomography (PET).

Purpose of the Study:

• To design and synthesize novel fatty acid analogs for 62Cu labeling.
• To evaluate the radiolabeling efficiency and myocardial uptake of these analogs.

Main Methods:

• Synthesis of two dithiosemicarbazone (DTS)-containing fatty acid analogs: FA-DTS and PFA-DTS.
• Radiolabeling with 64Cu (as a surrogate for 62Cu) acetate.
• In vivo biodistribution studies in mice to assess myocardial uptake and retention.

Main Results:

• Both FA-DTS and PFA-DTS were successfully synthesized.
• Simple, rapid, and quantitative radiolabeling with 64Cu was achieved.
• In vivo studies demonstrated significant distribution and retention of the radiolabeled compounds in the myocardium.

Conclusions:

• The synthesized fatty acid analogs are suitable for 62Cu labeling.
• These analogs show promising myocardial uptake and retention for potential PET imaging.
• Further development of these 62Cu-labeled fatty acid analogs is warranted.