Reusable nanobiocatalysts for the efficient extraction of pigments from orange peel

Area of Science:

• Biotechnology
• Green Chemistry
• Food Science

Background:

• Orange peels are a rich source of valuable carotenoid pigments.
• Traditional extraction methods often lack efficiency and sustainability.
• Nanobiocatalysis offers a promising avenue for improved extraction processes.

Purpose of the Study:

• To develop and evaluate nanobiocatalysts for enhanced carotenoid extraction from Nagpur mandarin orange peels.
• To investigate the immobilization of cellulase and pectinase onto magnetic nanoparticles (MNPs).
• To determine optimal process conditions for pigment extraction.

Main Methods:

• Cellulase and pectinase were immobilized onto magnetic nanoparticles (Fe3O4, 40-90 nm) using APTES and glutaraldehyde.
• Treated orange peels were extracted using organic solvents.
• Dinitrosalicylic acid assays were employed to assess peel hydrolysis.
• Optimization of pH (5.0) and temperature (50 °C) was performed.

Main Results:

• An 8-9 fold increase in carotenoid pigment extraction was achieved compared to free enzymes.
• The developed nanobiocatalysts demonstrated reusability over three cycles with a minimal yield drop (15%).
• Superior peel hydrolysis correlated with the highest extent of pigment extraction.

Conclusions:

• Immobilized cellulase and pectinase on magnetic nanoparticles represent an efficient and reusable system for carotenoid extraction from orange peels.
• This nanobiocatalytic approach offers a sustainable and effective method for valorizing citrus waste.
• The findings support the potential of nanobiotechnology in the food and nutraceutical industries.